Fiber-based macroscale fluorescence lifetime imaging for real-time in situ tissue diagnostics (Conference Presentation)

Joao Lagarto; Vladislav Shcheslavskiy; Francesco S. Pavone; Riccardo Cicchi

doi:10.1117/12.2543010

9 March 2020 Fiber-based macroscale fluorescence lifetime imaging for real-time in situ tissue diagnostics (Conference Presentation)

Joao Lagarto, Vladislav Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi

Author Affiliations +

Proceedings Volume 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis; 112340Q (2020) https://doi.org/10.1117/12.2543010
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The inability to efficiently separate autofluorescence signals from bright background illumination is a key issue that hinders widespread deployment of optical techniques such as time-correlated single photon counting (TCSPC) in clinical settings. We introduce a novel approach to TCSPC measurements that synchronizes the fluorescence acquisition with an externally modulated light source at 50 Hz, thus guaranteeing background-free fluorescence detection and “continuous” eye-perceived specimen illumination. A color camera synchronized with fluorescence detection provides spatial resolution to the single-point measurements, to generate autofluorescence lifetime maps in real-time. We believe this method provides a new research pathway for clinical translation of optical techniques.

Conference Presentation

Citation Download Citation

Joao Lagarto, Vladislav Shcheslavskiy, Francesco S. Pavone, and Riccardo Cicchi "Fiber-based macroscale fluorescence lifetime imaging for real-time in situ tissue diagnostics (Conference Presentation)", Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 112340Q (9 March 2020); https://doi.org/10.1117/12.2543010

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