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6 March 2020 Real time monitoring of daily variation of circulating tumor cells by in vivo flow cytometry (Conference Presentation)
Xi Zhu, Qi Liu, Xunbin Wei
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Proceedings Volume 11241, Biophotonics and Immune Responses XV; 112410E (2020) https://doi.org/10.1117/12.2544813
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Circulating tumor cells (CTCs) are considered as the “seeds” which disseminate from the solid tumors, circulate within the vasculature and colonize the distant organs (“soil”). CTCs level in peripheral blood has been used for diagnosis, staging, prognosis of cancer, as well as treatment evaluation. Currently, the ex vivo CTC isolation methods (e.g. CellSearch system) are limited to several milliliters of blood and do not take into account the temporal distribution of CTCs in the peripheral blood, However, few studies were conducted to investigate whether CTCs were released into the blood vessel erratically and whether the distribution of CTCs in the blood is uniform. A reliable research tool that can provide sufficient quantity of CTCs, as well as the temporal information of each CTC is necessary to study the temporal distribution of CTCs. In vivo flow cytometry (IVFC) has been demonstrated to be a powerful tool for real-time and continuous monitoring of circulating cells. The time points of each CTC events are recorded with IVFC detection. Here, we established an orthotopic mouse model of prostate cancer by using GFP labeled PC3 cell lines and monitored the variation of CTCs in mouse ear arteries during 24-h of a day with IVFC. Our results suggested that the temporal distribution of CTCs was not even in the orthotopic mouse model of prostate cancer and CTC release may be regulated by circadian rhythm. Our work is thus hopeful to provide a guideline on the time of blood sampling and gain insight into the shedding process of CTCs.
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Xi Zhu, Qi Liu, and Xunbin Wei "Real time monitoring of daily variation of circulating tumor cells by in vivo flow cytometry (Conference Presentation)", Proc. SPIE 11241, Biophotonics and Immune Responses XV, 112410E (6 March 2020); https://doi.org/10.1117/12.2544813
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KEYWORDS
Tumors
Blood
Flow cytometry
In vivo imaging
Mouse models
Prostate cancer
Blood vessels
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