Multi-wavelength diffractive beam shaper for rectangular flattop spots in flow cytometer (Conference Presentation)

Jingjing Zhao; Yong Han; Zeheng Jiao; Zixi Chao; Attila Tárnok; Zheng You

doi:10.1117/12.2544461

9 March 2020 Multi-wavelength diffractive beam shaper for rectangular flattop spots in flow cytometer (Conference Presentation)

Jingjing Zhao, Yong Han, Zeheng Jiao, Zixi Chao, Attila Tárnok, Zheng You

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430U (2020) https://doi.org/10.1117/12.2544461
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Flow cytometer is a powerful tool for the quantitative analysis of a large population of cells. A key factor determining the measurement accuracy and system stability is the illumination beam. The conventional beam shaper is composed of multiple geometrical optical elements, which should be precisely calibrated to produce elliptical Gaussian beams. It is difficult to control the sizes and positions of the focal spots formed by different wavelengths. A new beam shaping method based on diffractive optical element (DOE) is developed for rectangular flattop spots with multi-wavelength illumination. Its benefits include high simplicity, design flexibility, uniform illumination, and multifunctionality.

Conference Presentation

Citation Download Citation

Jingjing Zhao, Yong Han, Zeheng Jiao, Zixi Chao, Attila Tárnok, and Zheng You "Multi-wavelength diffractive beam shaper for rectangular flattop spots in flow cytometer (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430U (9 March 2020); https://doi.org/10.1117/12.2544461

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KEYWORDS

Beam shaping

Diffractive optical elements

Calibration

Gaussian beams

Microfluidics

Optical components

Precision calibration

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