Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy; Jonathan Nylk; Federico M. Gasparoli; Adrià Escobet-Montalbán; Anders K. Hansen; Dominik Marti; Peter E. Andersen; Kishan Dholakia

doi:10.1117/12.2544205

9 March 2020 Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy, Jonathan Nylk, Federico M. Gasparoli, Adrià Escobet-Montalbán, Anders K. Hansen, Dominik Marti, Peter E. Andersen, Kishan Dholakia

Author Affiliations +

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 112450J (2020) https://doi.org/10.1117/12.2544205
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.

Conference Presentation

Citation Download Citation

Madhu Veettikazhy, Jonathan Nylk, Federico M. Gasparoli, Adrià Escobet-Montalbán, Anders K. Hansen, Dominik Marti, Peter E. Andersen, and Kishan Dholakia "Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450J (9 March 2020); https://doi.org/10.1117/12.2544205

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Microscopy

Optical filtering

Tissue optics

Beam shaping

Aberration correction

Absorption

Gold

Show All Keywords

Keywords/Phrases

Search In:

Publication Years