Lattice light-sheet and Fresnel incoherent correlation holography

Mariana C. Potcoava; Shelagh Rodriguez; Zack Zurawski; Jon Art; Simon Alford

doi:10.1117/12.2545336

17 February 2020 Lattice light-sheet and Fresnel incoherent correlation holography

Mariana C. Potcoava, Shelagh Rodriguez, Zack Zurawski, Jon Art, Simon Alford

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124805 (2020) https://doi.org/10.1117/12.2545336
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We demonstrate Lattice Light-Sheet microscopy combined with dual-lens Fresnel Incoherent Correlation Holography (LLS-FINCH) in the detection path. We will describe the system alignment steps, provide an experimental demonstration, and provide the system characteristics in comparison to those of conventional LLS (glass-optics based). We present the imaging performance of the microscope and the adaptive optics module with an example of post-data-acquisition image reconstruction which includes an adaptive optics correction process.

Citation Download Citation

Mariana C. Potcoava, Shelagh Rodriguez, Zack Zurawski, Jon Art, and Simon Alford "Lattice light-sheet and Fresnel incoherent correlation holography", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124805 (17 February 2020); https://doi.org/10.1117/12.2545336

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