Raman hyperspectral imaging of transferrin-bound iron in T47D and MDA-MB-231 breast cancer cells

Ting Chean Khoo; Kate Tubbesing; Alena Rudkouskaya; Georgios Athanassiadis; Anna Sharikova; Margarida Barroso; Alexander Khmaladze

doi:10.1117/12.2547638

20 February 2020 Raman hyperspectral imaging of transferrin-bound iron in T47D and MDA-MB-231 breast cancer cells

Ting Chean Khoo, Kate Tubbesing, Alena Rudkouskaya, Georgios Athanassiadis, Anna Sharikova, Margarida Barroso, Alexander Khmaladze

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511E (2020) https://doi.org/10.1117/12.2547638
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Iron is an essential element required for human life, and is highly regulated in the body. Iron deficiency leads to many adverse health effects, such as anemias. The exact mechanisms of iron release in cells are not well known. We developed a Raman micro-spectroscopy technique that allows detection of transferrin (Tf) bound iron inside intact human cells. Ferric iron (Fe³⁺) bound to serum Tf is internalized into cells via the transferrin receptor (TfR). Methods that allow determining when and where Tf releases iron inside a cell lead to a better understanding of disease progression, including cancer. We have previously shown that Raman micro-spectroscopy is able to detect and quantify the Tf-bound iron in human breast cancer T47D cells. In this work, we applied hyperspectral Raman imaging to visualize the spatial distribution of Tf-bound iron in human breast cancer T47D and MDAMB231 cells internalized with iron-loaded Tf. We have also shown that Raman imaging can quantify the amount of iron under different times of Tf internalization prior to fixation. Raman microspectroscopy provides a unique way to determine the amount of iron under different Tf internalization times by employing the Raman metric, which was used to quantify iron content in iron bound Tf (holo-Tf) samples. Importantly, Raman microspectroscopy can be used to follow iron release from Tf in breast cancer cells. Determining the kinetics and location of iron release in cancer cells is key to further our understanding of iron metabolism during cancer progression.

Conference Presentation

Citation Download Citation

Ting Chean Khoo, Kate Tubbesing, Alena Rudkouskaya, Georgios Athanassiadis, Anna Sharikova, Margarida Barroso, and Alexander Khmaladze "Raman hyperspectral imaging of transferrin-bound iron in T47D and MDA-MB-231 breast cancer cells", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511E (20 February 2020); https://doi.org/10.1117/12.2547638

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available