High-speed super-multiplex organelle imaging

Jingwen Shou; Fanghao Hu; Robert Oda; Wei Min; Yasuyuki Ozeki

doi:10.1117/12.2544808

21 February 2020 High-speed super-multiplex organelle imaging

Jingwen Shou, Fanghao Hu, Robert Oda, Wei Min, Yasuyuki Ozeki

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112521C (2020) https://doi.org/10.1117/12.2544808
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Among various optical methods, fluorescence imaging has been the most widely exploited thanks to its superior sensitivity and specificity, but the resolvable colors are restricted to 2-5 colors because of the intrinsically broad and featureless spectra. Recently, this fluorescent “color barrier” was broken and super-multiplex optical imaging became possible taking advantage of well-designed Raman probes. However, the acquisition of the super-multiplex images is still relatively slow which impedes wider applications. Here, we demonstrate fast super-multiplex organelle imaging with high-speed color switching and acquisition, which accelerates the imaging speed by 2 orders of magnitude. We applied it in imaging cytometry, tracing mitosis and fast organelle motions in live cells. We anticipate that high-speed supermultiplex optical imaging can expand to a much wider field of biological researches.

Conference Presentation

Citation Download Citation

Jingwen Shou, Fanghao Hu, Robert Oda, Wei Min, and Yasuyuki Ozeki "High-speed super-multiplex organelle imaging", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521C (21 February 2020); https://doi.org/10.1117/12.2544808

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