Engineering functional nanoparticles for delivery in cells

Konstantina Alexaki; Maria Eleni Kyriazi; Afaf H. El Sagheer; Tom Brown; Antonios G. Kanaras

doi:10.1117/12.2538470

21 February 2020 Engineering functional nanoparticles for delivery in cells

Konstantina Alexaki, Maria Eleni Kyriazi, Afaf H. El Sagheer, Tom Brown, Antonios G. Kanaras

Proceedings Volume 11255, Colloidal Nanoparticles for Biomedical Applications XV; 112550K (2020) https://doi.org/10.1117/12.2538470
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The ability of DNA functionalised gold nanoparticles (AuNPs) to detect specific targets in vitro and in vivo has led to their development as suitable tools for sensing applications. However, endosomal entrapment is a common barrier in various nanoparticle delivery approaches. In this work, we present a new design strategy with the aim to enhance endosomal escape of DNA-coated AuNPs via the incorporation of a peptide that has been found to promote effective escape within cells. AuNPs are firstly modified with thiol terminated DNA strands followed by further surface functionalisation with cysteine terminated peptides. We show that optimized loading of peptides following DNA nanoparticle functionalisation of nanoparticles is feasible. DNA-peptide-coated AuNP hybrids show similar stability towards degradation by endocellular enzymes and similar specificity towards the detection of specific mRNA targets.

Citation Download Citation

Konstantina Alexaki, Maria Eleni Kyriazi, Afaf H. El Sagheer, Tom Brown, and Antonios G. Kanaras "Engineering functional nanoparticles for delivery in cells", Proc. SPIE 11255, Colloidal Nanoparticles for Biomedical Applications XV, 112550K (21 February 2020); https://doi.org/10.1117/12.2538470

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available