Detection of methylated cytosine on single strand DNA based on immune-functionalized and glutaraldehyde modified liquid exfoliated graphene field effect transistor

Jizhao Zhang; Shihiu Hu; Zhongrong Wang; Yunfang Jia

doi:10.1117/12.2559509

31 December 2019 Detection of methylated cytosine on single strand DNA based on immune-functionalized and glutaraldehyde modified liquid exfoliated graphene field effect transistor

Jizhao Zhang, Shihiu Hu, Zhongrong Wang, Yunfang Jia

Author Affiliations +

Proceedings Volume 11384, Eleventh International Conference on Signal Processing Systems; 113841D (2019) https://doi.org/10.1117/12.2559509
Event: Eleventh International Conference on Signal Processing Systems, 2019, Chengdu, China

Abstract

Detection of the 5-methylcytosine (5mC) is one of the requirements of modern epigenetics research. The main challenges in this issue are how to distinguish the 5mC site from the unmethylated ones in the chain of nucleotides, and determine its amount. To streamline the tedious operations in traditional bisulfite conversion (BC) and polymerase chain reaction (PCR) based detection methods, lots of graphene derivatives and electrochemistry (EC) sensors have been exploited. In this work, we would like to propose an electronic method for DNA methylation detection by using five testing single strand DNA (ssDNA) chains as proof-of-concepts, based on the glutaraldehyde modified liquid exfoliated graphene field effect transistor (LEG-FET). First of all, for the sake of identifying the 5mC site, the immunorecognition strategy is utilized and incorporated with LEG-FET working principle. That is, the methylation sites on testing ssDNA chains are first recognized by the fixed 5mC’s antibody (5mCab) molecules on the channel of LEG-FET, then they are transduced to the varied current between the electrodes of drain and source (I_DS). It is found, the changing ratios of I_DS (ΔI_DS/I^DS0) are in negative relation with the amount of 5mC sites (N_mC) at each of the ssDNA concentrations (C_ssDNA). When C_ssDNA is varied from 1 to 10⁶ pM, the slopes of the responding curves -ΔI_DS/I_DS0 vs. N_mC are increased from 0.54 to 3.70 %/N_mC; meanwhile, at each of constant N_mc, the slopes of -ΔI_DS/I_DS0 vs. C_ssDNA are also examined to proof the repeatability in DNA methylation detection.

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Jizhao Zhang, Shihiu Hu, Zhongrong Wang, and Yunfang Jia "Detection of methylated cytosine on single strand DNA based on immune-functionalized and glutaraldehyde modified liquid exfoliated graphene field effect transistor", Proc. SPIE 11384, Eleventh International Conference on Signal Processing Systems, 113841D (31 December 2019); https://doi.org/10.1117/12.2559509

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KEYWORDS

Graphene

Field effect transistors

Liquids

Electrodes

Molecules

Polymers

Sensors

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