Biophysical fingerprinting of single bacterial spores using laser Raman optical tweezers

Tobias Dahlberg; Dmitry Malyshev; Per Ola Andersson; Magnus Andersson

doi:10.1117/12.2558102

27 April 2020 Biophysical fingerprinting of single bacterial spores using laser Raman optical tweezers

Tobias Dahlberg, Dmitry Malyshev, Per Ola Andersson, Magnus Andersson

Proceedings Volume 11416, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXI; 114160I (2020) https://doi.org/10.1117/12.2558102
Event: SPIE Defense + Commercial Sensing, 2020, Online Only

Abstract

Spore-forming bacteria that cause diseases pose a danger in our society. When in spore form, bacteria can survive high temperatures and resist a plethora of disinfection chemicals. Effective disinfection approaches are thus critical. Since a population of bacterial spores is heterogeneous in many aspects, single spore analyzing methods are suitable when heterogeneous information cannot be neglected. We present in this work a highresolution Laser Raman optical tweezers that can trap single spores and characterize their Raman spectra. We first evaluate our system by measuring Raman spectra of spores, and purified DNA and DPA. Thereafter, we expose Bacillus thuringiensis spores to peracetic acid, chlorine dioxide, and sodium hypochlorite, which are common disinfection chemicals. The data reveals how these agents change the constitutes of a spore over time, thus improving on the mode of action of these disinfection chemicals.

Citation Download Citation

Tobias Dahlberg, Dmitry Malyshev, Per Ola Andersson, and Magnus Andersson "Biophysical fingerprinting of single bacterial spores using laser Raman optical tweezers", Proc. SPIE 11416, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXI, 114160I (27 April 2020); https://doi.org/10.1117/12.2558102

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