Temperature Dependences Of The Infrared And Circular Dichroism Spectra Of Ribonuclease A And Troponin C

Tatsuyuki Yamamoto; Masaru Tanokura; Mitsuo Tasumi

doi:10.1117/12.969455

1 December 1989 Temperature Dependences Of The Infrared And Circular Dichroism Spectra Of Ribonuclease A And Troponin C

Tatsuyuki Yamamoto, Masaru Tanokura, Mitsuo Tasumi

Proceedings Volume 1145, 7th Intl Conf on Fourier Transform Spectroscopy; (1989) https://doi.org/10.1117/12.969455
Event: Seventh International Conference on Fourier and Computerized Infrared Spectroscopy, 1989, Fairfax, VA, United States

Abstract

Temperature dependences of the infrared and CD spectra of bovine pancreatic ribonuclease A and bullfrog skeletal muscle troponin C have been examined. In order to extract information on the secondary structure changes of these proteins from their infrared spectra, the methods of difference spectrum and Fourier-self-deconvolution have been applied. Both the infrared and circular dichroism spectra have confirmed that ribonuclease A undergoes thermal denaturation at 60°C in a typical manner. On the contrary, calcium-free troponin C shows gradual spectral changes with increasing temperature, indicating the absence of drastic conformational changes. No denaturation seems to take place for calcium-bound toponin C even at 80°C.

Citation Download Citation

Tatsuyuki Yamamoto, Masaru Tanokura, and Mitsuo Tasumi "Temperature Dependences Of The Infrared And Circular Dichroism Spectra Of Ribonuclease A And Troponin C", Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); https://doi.org/10.1117/12.969455

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