Line-scan hyperspectral imaging microscopy with structured illumination

Xingchen Dong; Zhendong Li; Jie Dong; Kun Wang; Michael H. Köhler; Martin Jakobi; Alexander W. Koch

doi:10.1117/12.2565944

20 August 2020 Line-scan hyperspectral imaging microscopy with structured illumination

Xingchen Dong, Zhendong Li, Jie Dong, Kun Wang, Michael H. Köhler, Martin Jakobi, Alexander W. Koch

Proceedings Volume 11508, Unconventional Imaging and Adaptive Optics 2020; 115080U (2020) https://doi.org/10.1117/12.2565944
Event: SPIE Optical Engineering + Applications, 2020, Online Only

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Abstract

Hyperspectral imaging microscopy is a powerful analytical tool for spatial identification and spectral feature extraction in chemical and biological complex systems. Inspired by super-resolution microscopy, structured programmable projection coupled with spectral image reconstruction techniques is employed to improve the spatial resolution of spectroscopic imaging microscopy. In this work, a line-scan hyperspectral imaging microscope implemented with a digital light projector (DLP) was demonstrated. The DLP with a digital micromirror device (DMD) was used to project sinusoidal fringes with three angular orientations and three phase shifts. After synchronization of fringe projection, stage movement, and image acquisition, hyperspectral data sets were acquired, and image reconstruction was conducted using the nine-frame images for improved spatial resolution over the full wavelength range. This work contributes to the progress in microscale and nanoscale imaging using line-scan hyperspectral microscopy.

Citation Download Citation

Xingchen Dong, Zhendong Li, Jie Dong, Kun Wang, Michael H. Köhler, Martin Jakobi, and Alexander W. Koch "Line-scan hyperspectral imaging microscopy with structured illumination", Proc. SPIE 11508, Unconventional Imaging and Adaptive Optics 2020, 115080U (20 August 2020); https://doi.org/10.1117/12.2565944

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