Imaging of the corneal nerves ex vivo and in vivo using genetically-encoded calcium indicators

Matthew T. McPheeters; Brecken J. Blackburn; William J. Dupps; Andrew M. Rollins; Michael W. Jenkins

doi:10.1117/12.2578018

5 March 2021 Imaging of the corneal nerves ex vivo and in vivo using genetically-encoded calcium indicators

Matthew T. McPheeters, Brecken J. Blackburn, William J. Dupps, Andrew M. Rollins, Michael W. Jenkins

Proceedings Volume 11623, Ophthalmic Technologies XXXI; 116230T (2021) https://doi.org/10.1117/12.2578018
Event: SPIE BiOS, 2021, Online Only

Abstract

The morphology and histology of the corneal nerves in health and disease has been extensively studied, however, the function of these nerves has only been studied in a limited fashion with electrophysiology of the ciliary nerves or calcium reporter dyes in ex vivo corneas. Here, we present non-contact methods for imaging the genetically encoded calcium indicator GCaMP6f in murine corneal nerves both in situ in ex vivo globes and in vivo. These tools have great potential to improve understanding of diseases of the corneal nerves and the development of therapies for diminished neural function.

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Citation Download Citation

Matthew T. McPheeters, Brecken J. Blackburn, William J. Dupps, Andrew M. Rollins, and Michael W. Jenkins "Imaging of the corneal nerves ex vivo and in vivo using genetically-encoded calcium indicators", Proc. SPIE 11623, Ophthalmic Technologies XXXI, 116230T (5 March 2021); https://doi.org/10.1117/12.2578018

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