Deep-learning-based whole-brain imaging at single-neuron resolution

Kefu Ning; Xiaoyu Zhang; Xuefei Gao; Tao Jiang; He Wang; Siqi Chen; Anan Li; Jing Yuan

doi:10.1117/12.2582870

5 March 2021 Deep-learning-based whole-brain imaging at single-neuron resolution

Kefu Ning, Xiaoyu Zhang, Xuefei Gao, Tao Jiang, He Wang, Siqi Chen, Anan Li, Jing Yuan

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 1162928 (2021) https://doi.org/10.1117/12.2582870
Event: SPIE BiOS, 2021, Online Only

Abstract

Obtaining fine structures in the whole brain is necessary for understanding brain function. Simple and effective methods for large-scale 3D imaging at optical resolution are still lacking. Here, we proposed a deep-learning-based fluorescence micro-optical sectioning tomography (DL-fMOST) method for fast, high-resolution whole-brain imaging. We utilized a wide-field microscope and a convolutional neural network for optical sectioning imaging, replacing traditional optical method. A 3D dataset of a mouse brain with a voxel size of 0.32 × 0.32 × 2 µm was acquired in 1.5 days. We demonstrated the robustness of DL-fMOST for mouse brains with labeling of different types of neurons.

Conference Presentation

Citation Download Citation

Kefu Ning, Xiaoyu Zhang, Xuefei Gao, Tao Jiang, He Wang, Siqi Chen, Anan Li, and Jing Yuan "Deep-learning-based whole-brain imaging at single-neuron resolution", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 1162928 (5 March 2021); https://doi.org/10.1117/12.2582870

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Image resolution

Brain

Neurons

Microscopes

Neuroimaging

Neuroscience

Optical imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years