Characterization of laser microbiopsy for sub-microliter tissue sampling

Jason B. King; Nitesh Katta; James W. Tunnell; Thomas E. Milner

doi:10.1117/12.2577258

5 March 2021 Characterization of laser microbiopsy for sub-microliter tissue sampling

Jason B. King, Nitesh Katta, James W. Tunnell, Thomas E. Milner

Proceedings Volume 11631, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIX; 1163111 (2021) https://doi.org/10.1117/12.2577258
Event: SPIE BiOS, 2021, Online Only

Abstract

Tissue sampling is required for disease diagnostics and research. Traditional tissue sampling tools often remove more tissue than required, causing unnecessary pain and morbidity. We report a minimally invasive laser microbiopsy system to sample sub-microliter (<1mm³ ) tissue sections. A focused annular beam cuts conical-shaped tissue sections. Compatible diagnostic methods may include standard histopathology, rapid point-of-care microscopy, and genetic testing. An objective of the approach is to harvest a minimal volume while limiting damage to retain tissue integrity for diagnosis. The laser microbiopsy system consists of a Ho:YAG laser (Lumenis P120), a ZnSe aspheric collimator (ISP optics, f = 50.8 mm), two fused silica axicons (Thorlabs, α = 20°), and an aspheric focusing lens (ISP optics, f = 25.4 mm). The system is characterized to evaluate the required laser dosimetry, range of harvested tissue volumes, and residual thermal damage. We first characterized the system in silico using raytracing (Zemax) and a heat transfer modeling. We then characterized the system experimentally performing micro-biopsies in ex vivo porcine skin. Raytracing and experimental measurements show agreement in annular beam shape with an inner and outer radius of 300 μm and 400 μm near the beam focus. Experimental results show ability to harvest tissue sections ranging from 0.009 to 0.10 mm3 using pulse energies from 1 - 2 J.

Conference Presentation

Citation Download Citation

Jason B. King, Nitesh Katta, James W. Tunnell, and Thomas E. Milner "Characterization of laser microbiopsy for sub-microliter tissue sampling", Proc. SPIE 11631, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIX, 1163111 (5 March 2021); https://doi.org/10.1117/12.2577258

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available