Quantification of tryptophan in different layers of oral cancer tissue using fluorescence polarization technique

Einstein Gnanatheepam; Anandh Sundaramoorthy; Kanniyappan Udayakumar; Dornadula Koteeswaran; Singaravelu Ganesan; Aruna Prakasarao; Ganesan Singaravelu

doi:10.1117/12.2583352

5 March 2021 Quantification of tryptophan in different layers of oral cancer tissue using fluorescence polarization technique

Einstein Gnanatheepam, Anandh Sundaramoorthy, Kanniyappan Udayakumar, Dornadula Koteeswaran, Singaravelu Ganesan, Aruna Prakasarao, Ganesan Singaravelu

Author Affiliations +

Proceedings Volume 11636, Optical Biopsy XIX: Toward Real-Time Spectroscopic Imaging and Diagnosis; 116360G (2021) https://doi.org/10.1117/12.2583352
Event: SPIE BiOS, 2021, Online Only

Abstract

Oral cancer has a poor five-year survival rate and has not improved much in the past two decades which is due to late diagnosis. In current clinical practice analysis of Haematoxylin Eosin stained tissue biopsy is considered as a golden standard which is rather painful and routine check is not possible. In this regard, native fluorescence spectroscopy has been considered to discriminate cancer tissue based on relative alterations in the level of tryptophan. To estimate relative variations of tryptophan at different layers of tissue fluorescence polarization gating technique has been adopted which is based on the principle that the light from the superficial layer of tissue partially retain the polarization plane of incident light as they are less scattered while light from the deeper layer is completely depolarized due to multiple scattering. Integrated intensity of tryptophan was quantified, and subsequent statistical analysis has been carried out to evaluate the diagnostic potentiality of the proposed technique. It was found that the fractional variation of tryptophan in the superficial layer to the deeper layer was found to be statistically more significant in discriminating oral cancer than cumulative tryptophan in both layers.

Citation Download Citation

Einstein Gnanatheepam, Anandh Sundaramoorthy, Kanniyappan Udayakumar, Dornadula Koteeswaran, Singaravelu Ganesan, Aruna Prakasarao, and Ganesan Singaravelu "Quantification of tryptophan in different layers of oral cancer tissue using fluorescence polarization technique", Proc. SPIE 11636, Optical Biopsy XIX: Toward Real-Time Spectroscopic Imaging and Diagnosis, 116360G (5 March 2021); https://doi.org/10.1117/12.2583352

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cancer

Polarization

Luminescence

Tissues

Tissue optics

Diagnostics

Fluorescence spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years