Label-free photoacoustic expansion histopathology with enhanced temporal and spatial resolution

Hyojin Kim; Jin Woo Baik; Jin Young Kim; Seungwan Jeon; Myeongjoo Son; Kyunghee Byun; Chulhong Kim

doi:10.1117/12.2577203

5 March 2021 Label-free photoacoustic expansion histopathology with enhanced temporal and spatial resolution

Hyojin Kim, Jin Woo Baik, Jin Young Kim, Seungwan Jeon, Myeongjoo Son, Kyunghee Byun, Chulhong Kim

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116420M (2021) https://doi.org/10.1117/12.2577203
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Ultraviolet photoacoustic microscopy (UV-PAM) provides label-free imaging of cell nuclei with the superior optical absorption of DNAs and RNAs. UV-PAM does not require any additional staining process to visualize the structures of tissues and cells. However, conventional UV-PAM is limited in imaging speed to be used in real pre-clinical and clinical situations. To overcome this limitation, we developed an UV-PAM adopting a high-speed scanning module. We demonstrated the applicability of the system as an intraoperative surgical margin assessment tool by imaging formalinfixed paraffine-embedded (FFPE) sections of human colon cancer tissues and comparing these results to conventional optical microscopic images. Additionally, three more types of cancerous tissue such as liver, uterus, and kidney were photoacoustically examined. The imaging results successfully showed the characteristic features of cancerous and normal tissues, allowing rapid diagnosis of cancer. UV-PAM has achieved sufficient spatial resolution to distinguish the individual nuclei in human tissue, but it is challenging to resolve the closely adjacent nuclei. Therefore, we applied tissue expansion technology, which enables nanoscale imaging of subcellular components beyond the resolution of optical microscopy, to our developed system. A mouse brain section was physically expanded by hydrogel-tissue hybridization, and consequently distances between cell nuclei in the tissue increased. We could separate densely distributed nuclei in the hippocampus of mouse brain better compared to conventional UV-PAM. In short, we developed a novel PA imaging system with the enhanced temporal and spatial resolution by combining fast scanning modality and tissue expansion technology with UV-PAM.

Conference Presentation

Citation Download Citation

Hyojin Kim, Jin Woo Baik, Jin Young Kim, Seungwan Jeon, Myeongjoo Son, Kyunghee Byun, and Chulhong Kim "Label-free photoacoustic expansion histopathology with enhanced temporal and spatial resolution", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116420M (5 March 2021); https://doi.org/10.1117/12.2577203

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available