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Fluorescence lifetime imaging (FLIM) has proved to be ideal for non-invasive and very sensitive investigations in clinical applications. In contrast to pure intensity-based imaging, the usage of the timing information lifts FLIM on another level enabling functional imaging. The most salient example is metabolic imaging e.g. to investigate melanoma lesion. Here we show the capability of metabolic FLIM of monitoring melanoma growth in a very early stage. We also present the results of a multiphoton FLIM study on tracing nanoparticles after topical application on human skin in vivo for safety aspects of cosmetics.
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Hauke Studier, Amy Holmes, Michael Pastore, Wolfgang H. Becker, Michael S. Roberts, "Functional imaging progress for clinical use," Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480F (5 March 2021); https://doi.org/10.1117/12.2578231