Translation of third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tumor tissue

Laura M. Van Huizen; Teodora Radonic; Frank van Mourik; Daniëlle Seinstra; Chris Dickhoff; Johannes M. A. Daniels; Idris Bahce; Jouke T. Annema; Marie Louise Groot

doi:10.1117/12.2578174

5 March 2021 Translation of third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tumor tissue

Laura M. Van Huizen, Teodora Radonic, Frank van Mourik, Daniëlle Seinstra, Chris Dickhoff, Johannes M. A. Daniels, Idris Bahce, Jouke T. Annema, Marie Louise Groot

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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480Y (2021) https://doi.org/10.1117/12.2578174
Event: SPIE BiOS, 2021, Online Only

Abstract

Third and second harmonic generation (THG/SHG) and two-photon autofluorescence (2PEF) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test a compact, portable ex-vivo THG/SHG/2PEF microscope (Femto Diagnostics B.V.) in the hospital. We will present the first results of an ongoing study in which we image lung tumor tissue immediately after excision, compare the images to the golden standard histopathology, assess the quality of diagnosis by independent pathologists, and examine the clinical impact of the THG/SHG/2PEF microscope. We also focus on identification and distinction of different immune cells.

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Laura M. Van Huizen, Teodora Radonic, Frank van Mourik, Daniëlle Seinstra, Chris Dickhoff, Johannes M. A. Daniels, Idris Bahce, Jouke T. Annema, and Marie Louise Groot "Translation of third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tumor tissue", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480Y (5 March 2021); https://doi.org/10.1117/12.2578174

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