Non-orthogonal dual-objective light-sheet microscopy for subcellular imaging of cleared tissues

Kevin Bishop; Robert Serafin; Philip R. Nicovich; Adam K. Glaser; Jonathan T. C. Liu

doi:10.1117/12.2578762

5 March 2021 Non-orthogonal dual-objective light-sheet microscopy for subcellular imaging of cleared tissues

Kevin Bishop, Robert Serafin, Philip R. Nicovich, Adam K. Glaser, Jonathan T. C. Liu

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164918 (2021) https://doi.org/10.1117/12.2578762
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Light-sheet microscopy (LSM) has emerged as the technique of choice for many biologists imaging large cleared tissues due to its speed and optical efficiency, which make it possible to generate massive datasets of large specimens at high resolution. Here, we build on several recent innovations in LSM to present a non-orthogonal dual-objective (NODO) LSM system with axial sweeping in an open-top configuration. This system is specifically designed to image large cleared brain tissues, such as for axonal connectomics, and provides subcellular resolution (0.3 µm lateral, 2 µm axial) of large cleared samples up to 8 mm thick.

Conference Presentation

Citation Download Citation

Kevin Bishop, Robert Serafin, Philip R. Nicovich, Adam K. Glaser, and Jonathan T. C. Liu "Non-orthogonal dual-objective light-sheet microscopy for subcellular imaging of cleared tissues", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164918 (5 March 2021); https://doi.org/10.1117/12.2578762

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