Exchangeable fluorophore labels in super-resolution fluorescence microscopy

Mike Heilemann

doi:10.1117/12.2584988

5 March 2021 Exchangeable fluorophore labels in super-resolution fluorescence microscopy

Mike Heilemann

Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 1165008 (2021) https://doi.org/10.1117/12.2584988
Event: SPIE BiOS, 2021, Online Only

Abstract

We present fluorophore labels that transiently and repetitively bind to their targets as probes for various types of super-resolution fluorescence microscopy. Transient labels typically show a weak affinity to a target, and exchange constantly with the buffer that constitutes a reservoir with a large amount of intact probes, leading to repetitive binding events to the same target (we refer to these labels as “exchangeable labels”). This dynamic labeling approach is insensitive to common photobleaching and yields a constant fluorescence signal over time, which has been successfully exploited in SMLM, STED, single-particle tracking and super-resolution optical fluctuation imaging (SOFI). We discuss properties of suitable exchangeable labels and experimental parameters for optimal performance for the different super-resolution methods. In addition, we show how to combine different classes of exchangeable labels for high-quality multicolor super-resolution imaging.

Conference Presentation

Citation Download Citation

Mike Heilemann "Exchangeable fluorophore labels in super-resolution fluorescence microscopy", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 1165008 (5 March 2021); https://doi.org/10.1117/12.2584988

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