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5 March 2021 Super resolution imaging of proteins’ organization and conformation in 3D using polarized single molecule localization
Valentina Curcio, Cesar Valades Cruz, Luis A. Alemán Castañeda, Manos Mavrakis, Miguel Alonso, Sophie Brasselet
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Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 116500H (2021) https://doi.org/10.1117/12.2578555
Event: SPIE BiOS, 2021, Online Only
Abstract
Measuring single molecules’ 3D orientations in addition to their 3D spatial localization is still today a challenge, due to the intrinsic coupling of both spatial and orientational parameters in the point spread function (PSF) image formation. We present polarized fluorescence microscopy methods able to report both orientational and spatial information from single molecules with high precision. These methods are applied to STORM and PALM nanoscale imaging of actin filaments organization and membrane proteins’ conformational changes in 3D.
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Valentina Curcio, Cesar Valades Cruz, Luis A. Alemán Castañeda, Manos Mavrakis, Miguel Alonso, and Sophie Brasselet "Super resolution imaging of proteins’ organization and conformation in 3D using polarized single molecule localization", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500H (5 March 2021); https://doi.org/10.1117/12.2578555
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KEYWORDS
Proteins
Molecules
Super resolution
3D image processing
Point spread functions
Microscopy
Polarization
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