Analysis of the number of size parameters that can be reliably estimated from cell scattering

Ashley E. Cannaday; Rajdeep Singh; James Hoelle

doi:10.1117/12.2577079

5 March 2021 Analysis of the number of size parameters that can be reliably estimated from cell scattering

Ashley E. Cannaday, Rajdeep Singh, James Hoelle

Proceedings Volume 11657, Biomedical Applications of Light Scattering XI; 1165704 (2021) https://doi.org/10.1117/12.2577079
Event: SPIE BiOS, 2021, Online Only

Abstract

Angular scattering can be used to obtain morphological information from biological specimen, such as the mean size of organelles. We will investigate the limitations that arise when fitting cell scattering to a Mie theory model and extracting organelle size estimates. Using simulation studies of the scattering of two organelle populations in a cell (mitochondria and lysosomes), we will determine under what conditions multiple size distribution parameters can be estimated. Additionally, the analysis method that will allow for the most accurate and smallest organelle size changes to be measured will be investigated computationally.

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Citation Download Citation

Ashley E. Cannaday, Rajdeep Singh, and James Hoelle "Analysis of the number of size parameters that can be reliably estimated from cell scattering", Proc. SPIE 11657, Biomedical Applications of Light Scattering XI, 1165704 (5 March 2021); https://doi.org/10.1117/12.2577079

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