Application of the spectrofluorimetric research method to study the complexes «Pt NPs -platelets»

Karina I. Matveeva; Andrey Y. Zyubin; E. A. Demishkevich; V. V. Rafalskiy; E. M. Moiseeva; I. I. Kon; A. A. Kundalevich; V. V. Butova; I. G. Samusev

doi:10.1117/12.2602524

9 October 2021 Application of the spectrofluorimetric research method to study the complexes «Pt NPs -platelets»

Karina I. Matveeva, Andrey Y. Zyubin, E. A. Demishkevich, V. V. Rafalskiy, E. M. Moiseeva, I. I. Kon, A. A. Kundalevich, V. V. Butova, I. G. Samusev

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Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119003I (2021) https://doi.org/10.1117/12.2602524
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

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Abstract

This paper describes a detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs). Fluorescence, quantum yield, and platelet amino acid lifetime changes in the presence and without femtosecond ablated platinum NPs have been studied. Fluorescence spectroscopy analysis of main fluorescent amino acids and their residues (tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe)) belonging to the platelet membrane have been performed. The possibility of energy transfer between Pt NPs and the platelet membrane has been revealed. Förster Resonance Energy Transfer (FRET) model was used to perform the quantitative evaluation of energy transfer parameters. The prospects of Pt NPs usage deals with quenching-based sensing for pathology’s based on platelet conformations as cardiovascular diseases have been demonstrated.

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Karina I. Matveeva, Andrey Y. Zyubin, E. A. Demishkevich, V. V. Rafalskiy, E. M. Moiseeva, I. I. Kon, A. A. Kundalevich, V. V. Butova, and I. G. Samusev "Application of the spectrofluorimetric research method to study the complexes «Pt NPs -platelets»", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119003I (9 October 2021); https://doi.org/10.1117/12.2602524

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