Fluorescence tags using FRET networks and DNA structural change

Yusuke Ogura; Keita Hayashi; Jun Tanida

doi:10.1117/12.2615670

27 October 2021 Fluorescence tags using FRET networks and DNA structural change

Yusuke Ogura, Keita Hayashi, Jun Tanida

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250Q (2021) https://doi.org/10.1117/12.2615670
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Multiplex fluorescence imaging is an important technique for elucidating activities of cells through visualization of the behavior of biomolecules simultaneously. In this paper, we present a method for constructing a fluorescence tag library using Förster resonance energy transfer (FRET) networks and DNA nanostructures which can change in response to molecular input. This method enables us, for example, to detect an event of sensing a target molecule by receiving spectral signals from the FRET network. Calculation results demonstrate that use of DNA nanostructures is effective to switch the fluorescence spectrum generated from a fluorescence tag when sensing a target molecule. The diversity of spectral fingerprints of the tags is also verified. The method has capability in implementing a large sort of fluorescence tags for multiplex imaging.

Citation Download Citation

Yusuke Ogura, Keita Hayashi, and Jun Tanida "Fluorescence tags using FRET networks and DNA structural change", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250Q (27 October 2021); https://doi.org/10.1117/12.2615670

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