Multispectral time-resolved fluorescence microscopy based on compressive acquisitions

Alberto Ghezzi; Andrea Farina; Gianluca Valentini; Andrea Bassi; Cosimo D'Andrea

doi:10.1117/12.2607176

2 March 2022 Multispectral time-resolved fluorescence microscopy based on compressive acquisitions

Alberto Ghezzi, Andrea Farina, Gianluca Valentini, Andrea Bassi, Cosimo D'Andrea

Proceedings Volume 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; 1196602 (2022) https://doi.org/10.1117/12.2607176
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Multidimensional fluorescence microscopy techniques produce dataset rich of information (space, emission spectrum and lifetime) to investigate photophysical processes in biological samples. To acquire a 4D dataset, one promising microscope design is based on the single-pixel camera scheme and on compressive sensing acquisitions, thanks to which the measurement time can be reduced. Within this framework, a computational step is required to move from the acquisition space to the pixel space and, subsequently, the analysis can be carried out exploiting the high dimensionality. In this work we present an experimental system and a fast-fit method that can produce a map of fluorophore concentrations in parallel to the measurement routine.

Conference Presentation

Citation Download Citation

Alberto Ghezzi, Andrea Farina, Gianluca Valentini, Andrea Bassi, and Cosimo D'Andrea "Multispectral time-resolved fluorescence microscopy based on compressive acquisitions", Proc. SPIE 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, 1196602 (2 March 2022); https://doi.org/10.1117/12.2607176

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