High-speed multicolor stimulated Raman imaging

Yasuyuki Ozeki

doi:10.1117/12.2608050

3 March 2022 High-speed multicolor stimulated Raman imaging

Yasuyuki Ozeki

Proceedings Volume 11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; 1197305 (2022) https://doi.org/10.1117/12.2608050
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

In Raman imaging, improving the imaging speed is important in various applications such as large-scale imaging analysis and multiplex imaging, where imaging time restricts their applicability. Stimulated Raman scattering (SRS) microscopy has improved the speed of Raman imaging, while it is still restricted by several factors such as laser tuning time and signal-to-noise ratio. Here we introduce various approaches to break these barriers: ultrahigh-speed wavelength-switching of optical pulses for large-scale multicolor vibrational imaging of cells with SRS imaging flow cytometry, integrated SRS and fluorescence microscopy for super-multiplex time-lapse imaging and cytometry, and quantum enhancement to reduce the shot noise below the standard quantum limit.

Conference Presentation

Citation Download Citation

Yasuyuki Ozeki "High-speed multicolor stimulated Raman imaging", Proc. SPIE 11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, 1197305 (3 March 2022); https://doi.org/10.1117/12.2608050

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available