Disordered plasmonic substrate-based wide-field super-resolution imaging

Hongki Lee; Hajun Yoo; Woo Joong Rhee; Gwiyeong Moon; Changhun Lee; Jeon-Soo Shin; Donghyun Kim

doi:10.1117/12.2609401

3 March 2022 Disordered plasmonic substrate-based wide-field super-resolution imaging

Hongki Lee, Hajun Yoo, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, Donghyun Kim

Proceedings Volume 11978, Plasmonics in Biology and Medicine XIX; 119780A (2022) https://doi.org/10.1117/12.2609401
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We have investigated the feasibility of disordered plasmonic nanocomposites for super-resolution imaging. Annealing-based nanocomposite substrate has a great potential in biomedical optical and sensing technology because it can be mass-produced without difficult manufacturing processes. We introduce a new approach for wide-field super-resolution fluorescence imaging based on the nanocomposite island substrates, which we call nanospeckle illumination microscopy (NanoSIM). Near-field speckle patterns produced on disordered nanoisland substrates can help reconstruction of high-resolution fluorescence images with appropriate basis images. We have acquired basis images using azimuthal scanning illumination (ASI). Each ASI produces nonuniform nanoscale near-field speckles which can excite fluorescent dyes within a subdiffraction-limited area. While exploiting the random nature of plasmonic nanocomposite, NanoSIM does not require any specific polarization state to be maintained for ASI. We have tested NanoSIM to obtain super-resolved mages of molecules on the HeLa cell membrane. The full-width-at-half maximum was shown to improve by more than three times over the diffraction-limit with 360 basis images. Reconstructed images of gangliosides distribution on the HeLa cell suggest that fewer basis images may produce almost the same resolution with a shorter computation time. The optical resolution and sensitivity of disordered plasmonic substrate can be further enhanced by controlling the geometrical features of nanoislands structure.

Conference Presentation

Citation Download Citation

Hongki Lee, Hajun Yoo, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon-Soo Shin, and Donghyun Kim "Disordered plasmonic substrate-based wide-field super-resolution imaging", Proc. SPIE 11978, Plasmonics in Biology and Medicine XIX, 119780A (3 March 2022); https://doi.org/10.1117/12.2609401

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