Gold-coated tilted fiber Bragg gratings (TFBGs) have been extensively studied over the past years, particularly for biosensing purposes. Surface Plasmon Resonance (SPR) is generated through the deposition of a gold layer of appropriate thickness onto the grating region. The combination of SPR and TFBG permits to create a comb-like spectrum of narrow-band cladding mode resonances, which is usually demodulated by tracking the change of optical features of a selected peak. Here, for the first time to the best of our knowledge, a twenty-fold more sensitive demodulation technique based on the intersection of the upper and lower envelopes of gold-coated TFBG spectra is presented. This method has been successfully applied in biosensing for the detection of HER2 (Human Epidermal Growth Factor Receptor-2) proteins, a crucial breast cancer biomarker. Some practical improvements have also been proposed and assessed: first, a uniform FBG has been superimposed on the TFBG to reduce the read-out wavelength span to 10 nm instead of 70 nm, while keeping the temperature-compensated measurements; second, a microfluidic system has been designed and integrated to inject the samples towards the sensor at controlled flow rates. All these novelties make this sensing platform even more attractive and promising for use in practical applications.
|