In order to explore the applicability of biodiesel on marine diesel engines, this paper is based on the bench test of a sixcylinder medium-speed diesel engine, under the load conditions of 25%, 50%, 75%, 90% ,100%, respectively. Fuel 0# diesel and three kinds of biodiesel B5, B10, B15, analyze the vibration signals measured at the cylinder head, body, fuselage, The study found that the higher the content of biodiesel, the smaller the vibration response intensity of the cylinder head, and the piston side thrust generated by burning biodiesel is also significantly smaller than that generated by burning 0# diesel. After comparing the vibration intensity, it was found that the higher the biodiesel content, the smaller the vibration intensity of the cylinder head and the fuselage, and the greater the vibration intensity of the machine foot position. The vibration intensity of diesel engine is small under low load conditions of 25%, 50% and 75%, and large under high load conditions of 90% and 100%.
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