Multimodal microscopy imaging of cardiac collagen network: Are we looking at the same structures?

Zhao Zhang; Lily Neff; Amy D. Bradshaw; Hongming Fan; Emily Ye; William Richardson; Bruce Z. Gao; Tong Ye

doi:10.1117/12.2650223

14 March 2023 Multimodal microscopy imaging of cardiac collagen network: Are we looking at the same structures?

Zhao Zhang, Lily Neff, Amy D. Bradshaw, Hongming Fan, Emily Ye, William Richardson, Bruce Z. Gao, Tong Ye

Proceedings Volume 12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023; 1235508 (2023) https://doi.org/10.1117/12.2650223
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Visualization of collagen fibers in cardiac tissues is essential for clinical diagnosis and pathological analysis of cardiac fibrosis. Selecting a proper imaging method is still challenging for researchers and clinicians who want to determine specific information about the collagen network in cardiac tissues. We examined fibrillar collagen network from mouse ventricular myocardium by commonly available light microscopy techniques using our home-built multimodal microscope. Myocardial slices were unstained or stained with either Picrosirius red or collagen type I antibody/dye conjugation, then imaged by polarized light, confocal fluorescence, second harmonic generation (SHG), two-photon excited fluorescence (TPEF), and stimulated emission depletion (STED) microscopy techniques. This study is intended to serve as a reference for basic research and clinical evaluation of fibrillar collage network in cardiac tissues.

Conference Presentation

Citation Download Citation

Zhao Zhang, Lily Neff, Amy D. Bradshaw, Hongming Fan, Emily Ye, William Richardson, Bruce Z. Gao, and Tong Ye "Multimodal microscopy imaging of cardiac collagen network: Are we looking at the same structures?", Proc. SPIE 12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023, 1235508 (14 March 2023); https://doi.org/10.1117/12.2650223

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