Computational enhancement of lateral resolution using conventional fluorescent microscopes

Saeed Bohlooli Darian; Bjorn Paulson; Jeongmin Oh; Jun Ki Kim

doi:10.1117/12.2648219

15 March 2023 Computational enhancement of lateral resolution using conventional fluorescent microscopes

Saeed Bohlooli Darian, Bjorn Paulson, Jeongmin Oh, Jun Ki Kim

Proceedings Volume 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; 123830F (2023) https://doi.org/10.1117/12.2648219
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Understanding living cells and imaging intracellular dynamics requires complex and expensive devices. Conventional fluorescence microscopes suffer from poor resolution, while super-resolution comes with its own tradeoffs, making multi-cell studies challenging. We apply computational techniques such as super-resolution radial fluctuations to the tissue micrograph sequences, comparing a variety of numerical modifications and parameter settings to enable imaging of subcellular structures with higher resolution, reduced background noise, and enhanced signal intensity. Discrete organelle structures and dynamics are distinguishable in the final images, despite the original images having poor lateral resolution. The resultant computational techniques are widely available for use in studies anywhere

Citation Download Citation

Saeed Bohlooli Darian, Bjorn Paulson, Jeongmin Oh, and Jun Ki Kim "Computational enhancement of lateral resolution using conventional fluorescent microscopes", Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830F (15 March 2023); https://doi.org/10.1117/12.2648219

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