Enhanced resolution dispersion optimized multiphoton microscopy with spatial frequency modulation imaging

Daniel Scarbrough; Anna Thomas; Jeff Field; Randy Bartels; Jeff Squier

doi:10.1117/12.2650490

25 April 2023 Enhanced resolution dispersion optimized multiphoton microscopy with spatial frequency modulation imaging

Daniel Scarbrough, Anna Thomas, Jeff Field, Randy Bartels, Jeff Squier

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840O (2023) https://doi.org/10.1117/12.2650490
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Using the structured illumination, single pixel detection imaging technique SPatIal Frequency modulation Imaging (SPIFI), we demonstrate a cascaded Wavelength Domain and Spatial Domain (WD-SD-SPIFI) system enabling real-time, in-line, second order dispersion compensation optimization for multiphoton imaging. Enhanced resolution is demonstrated by imaging a sub-diffractive 140 nm fluorescent nanodiamond with Two Photon Excitation Fluorescence (2PEF) to measure the Point Spread Function (PSF). With a 1034 nm pulsed laser through a Numerical Aperture (NA) of 0.5, a PSF Full Width at Half Max (FWHM) of 780 nm was measured with minimal post processing analysis that only requires Fast Fourier Transforms (FFTs).

Citation Download Citation

Daniel Scarbrough, Anna Thomas, Jeff Field, Randy Bartels, and Jeff Squier "Enhanced resolution dispersion optimized multiphoton microscopy with spatial frequency modulation imaging", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840O (25 April 2023); https://doi.org/10.1117/12.2650490

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