Three-dimensional analysis of water dynamics in human skin by stimulated Raman scattering imaging

Takaha Mizuguchi; Christopher Takaya Knight; Makiko Goto; Masato Ninomiya; Mariko Egawa; Yasuyuki Ozeki

doi:10.1117/12.2647651

15 March 2023 Three-dimensional analysis of water dynamics in human skin by stimulated Raman scattering imaging

Takaha Mizuguchi, Christopher Takaya Knight, Makiko Goto, Masato Ninomiya, Mariko Egawa, Yasuyuki Ozeki

Proceedings Volume 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; 123920A (2023) https://doi.org/10.1117/12.2647651
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The stratum corneum (SC), the outermost layer of the skin, plays a vital role in providing a barrier against dry environments. In order to understand the barrier function and overall condition of the skin, it is essential to assess the ability of the SC to absorb and retain water. In this study, we used stimulated Raman scattering (SRS) imaging to investigate the threedimensional SC structure and water distribution as water was absorbed into dried SC sheets. Our findings indicate that the process of water absorption and retention is dependent on the specific sample and can be spatially heterogeneous. These results demonstrate the potential of SRS imaging in diagnosing skin conditions.

Conference Presentation

Citation Download Citation

Takaha Mizuguchi, Christopher Takaya Knight, Makiko Goto, Masato Ninomiya, Mariko Egawa, and Yasuyuki Ozeki "Three-dimensional analysis of water dynamics in human skin by stimulated Raman scattering imaging", Proc. SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, 123920A (15 March 2023); https://doi.org/10.1117/12.2647651

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