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12 March 2024 Comparing fluorescent contrast agents for fluorescence guided surgery using 3-D cryo-imaging
Augustino V. Scorzo, Caleb Y. Kwon, Rendall R. Strawbridge, Ryan B. Duke, William R. Warner, Kristen L. Chen, Chengpei Li, Xiaoyao Fan, David W. Roberts, Keith D. Paulsen, Scott C. Davis
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Proceedings Volume 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X; 1282504 (2024) https://doi.org/10.1117/12.3003221
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Fluorescence cryo-imaging is a high-resolution optical imaging technique that produces 3-D whole-body biodistributions of fluorescent molecules within an animal specimen. To accomplish this, animal specimens are administered a fluorescent molecule or reporter and are frozen to be autonomously sectioned and imaged at a temperature of -20°C or below. Thus, to apply this technique effectively, administered fluorescent molecules should be relatively invariant to low temperature conditions for cryo-imaging and ideally the fluorescence intensity should be stable and consistent in both physiological and cryo-imaging conditions. Herein, we assessed the mean fluorescence intensity of 11 fluorescent contrast agents as they are frozen in a tissue-simulating phantom experiment and show an example of a tested fluorescent contrast agent in a cryo-imaged whole pig brain. Most fluorescent contrast agents were stable within ~25% except for FITC and PEGylated FITC derivatives, which showed a dramatic decrease in fluorescence intensity when frozen.
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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Augustino V. Scorzo, Caleb Y. Kwon, Rendall R. Strawbridge, Ryan B. Duke, William R. Warner, Kristen L. Chen, Chengpei Li, Xiaoyao Fan, David W. Roberts, Keith D. Paulsen, and Scott C. Davis "Comparing fluorescent contrast agents for fluorescence guided surgery using 3-D cryo-imaging", Proc. SPIE 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X, 1282504 (12 March 2024); https://doi.org/10.1117/12.3003221
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KEYWORDS
Contrast agents
Fluorescence
Molecules
Animal model studies
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