Multimodal multiphoton dynamic imaging: determining the force field in the mouse paw

Anne Bias; Katrin J. Befeldt; Robert Günther; Anja E. Hauser; Raluca A. Niesner; Ingeborg E. Beckers

doi:10.1117/12.3001601

12 March 2024 Multimodal multiphoton dynamic imaging: determining the force field in the mouse paw

Anne Bias, Katrin J. Befeldt, Robert Günther, Anja E. Hauser, Raluca A. Niesner, Ingeborg E. Beckers

Proceedings Volume 12834, Multimodal Biomedical Imaging XIX; 1283403 (2024) https://doi.org/10.1117/12.3001601
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We hypothesize that strain of the synovial membrane of joints and surrounding tissues due to altered force ratios can be optically detected at cellular level in vivo to infer acting forces. This is crucial because immune cells adapt their function to disbalanced mechanical load. Time series of metacarpophalangeal joints in mouse paws are imaged using multimodal multiphoton microscopy. A three-dimensional method based on the Gaussian-Laplacian pyramid and an optical flow algorithm is used to determine morphological shift between consecutive time points, effectively overcoming image processing challenges. From this, the force field in bulk tissue is approximated based on Hooke's law.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Anne Bias, Katrin J. Befeldt, Robert Günther, Anja E. Hauser, Raluca A. Niesner, and Ingeborg E. Beckers "Multimodal multiphoton dynamic imaging: determining the force field in the mouse paw", Proc. SPIE 12834, Multimodal Biomedical Imaging XIX, 1283403 (12 March 2024); https://doi.org/10.1117/12.3001601

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