Can photobiomodulation be studied in vitro? Investigation on the interaction between cell culture medium and short-wavelength blue LED light

Giada Magni; Martina Banchelli; Leonardo Ciaccheri; Marina Mazzoni; Barbara Adinolfi; Lucia Cavigli; Roberto Pini; Francesca Rossi

doi:10.1117/12.3003677

12 March 2024 Can photobiomodulation be studied in vitro? Investigation on the interaction between cell culture medium and short-wavelength blue LED light

Giada Magni, Martina Banchelli, Leonardo Ciaccheri, Marina Mazzoni, Barbara Adinolfi, Lucia Cavigli, Roberto Pini, Francesca Rossi

Author Affiliations +

Proceedings Volume 12840, Optical Interactions with Tissue and Cells XXXV; 128400E (2024) https://doi.org/10.1117/12.3003677
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Photobiomodulation therapy is successfully applied in several medical fields, despite the fact that mechanisms underlying photobiomodulation are not yet fully clear. Cell cultures are one of the most exploited tools to study the processes that regulate photobiomodulation, even if they have some limitation. One of the main problem arising when studying the interaction of light with cell culture, is the interaction between the electromagnetic radiation and the cell culture medium. This interaction can limit the feasibility and reliability of the experimental results. In previous studies conducted on different cell types isolated from skin tissue, we have shown that low doses of blue LED light interact with cells, increasing the outward currents, viability, and proliferation, but without increasing the reactive oxygen species and damaging the mitochondria. These studies were performed following different experimental protocols, irradiating the cells both in the culture medium and without the medium. In order to further clarify our findings, we are now exploring the possible interactions between the medium for cell cultures (Dulbecco Modified Eagle’s Medium (DMEM)) and the electromagnetic radiation, irradiating medium samples with different doses of blue LED light (410-430 nm). The directly irradiated samples were then analyzed with Near-Infrared and Raman spectroscopy. Our results point out that the irradiated DMEM shows a a different spectral beahviour at high fluences (>40 J/cm2); moreover, these modifications are correlated to the riboflavin component currently used in the medium.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Giada Magni, Martina Banchelli, Leonardo Ciaccheri, Marina Mazzoni, Barbara Adinolfi, Lucia Cavigli, Roberto Pini, and Francesca Rossi "Can photobiomodulation be studied in vitro? Investigation on the interaction between cell culture medium and short-wavelength blue LED light", Proc. SPIE 12840, Optical Interactions with Tissue and Cells XXXV, 128400E (12 March 2024); https://doi.org/10.1117/12.3003677

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