ZOLA 3D, single molecule microscope technological transfer from research lab to ISO 9001 core facility

Yumi Gosselin; Mickael Lelek; Audrey Salles; Christophe Zimmer; Spencer Shorte; Elric Esposito

doi:10.1117/12.3002293

12 March 2024 ZOLA 3D, single molecule microscope technological transfer from research lab to ISO 9001 core facility

Yumi Gosselin, Mickael Lelek, Audrey Salles, Christophe Zimmer, Spencer Shorte, Elric Esposito

Proceedings Volume 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; 1284908 (2024) https://doi.org/10.1117/12.3002293
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Single Molecule Localization Microscopy (SMLM) and its ability to resolve < 100 nm structures has generated an evergrowing demand in biomedical research. This technique is highly relevant when trying to gain better understanding of biological structures details or cellular machinery in infectious models. The Imaging and Modelling Unit led by C. Zimmer developed an open optical and computational method based on Zernike Optimised Localisation Approach (ZOLA) enabling 3D localization of single molecules using point spread function (PSF) engineering in the detection path. This technique offers different performances and trade-off depending on the required application. This unique flexibility is relevant when dealing with various types of samples and models as those presented to an Imaging core facility. We will present how the Unit of Technology and Services (UTechS) Photonic Bio Imaging (PBI), the imaging platform of Institut Pasteur in Paris has conducted the technological transfer of ZOLA 3D from a research laboratory to a Bio Safety Level 2 (BSL2) ISO 9001 core facility. This will make flexible 3D super-resolution imaging accessible to a wide range of biological projects, including the study of pathogens.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yumi Gosselin, Mickael Lelek, Audrey Salles, Christophe Zimmer, Spencer Shorte, and Elric Esposito "ZOLA 3D, single molecule microscope technological transfer from research lab to ISO 9001 core facility", Proc. SPIE 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, 1284908 (12 March 2024); https://doi.org/10.1117/12.3002293

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KEYWORDS

Point spread functions

3D image processing

Biological imaging

Microscopes

3D modeling

Biological samples

Molecules

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