Enhancing signal-to-noise ratio in stimulated Brillouin microscopy with low duty cycle nanosecond pulses

Desmond M. Chow; Seok-Hyun Yun

doi:10.1117/12.2692239

12 March 2024 Enhancing signal-to-noise ratio in stimulated Brillouin microscopy with low duty cycle nanosecond pulses

Desmond M. Chow, Seok-Hyun Yun

Proceedings Volume 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; 128540D (2024) https://doi.org/10.1117/12.2692239
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stimulated Brillouin scattering (SBS) is a valuable technique for studying the mechanical properties of biological samples. We propose a novel scheme utilizing low duty cycle, nanosecond pulses for pump and probe beams. Our approach achieves higher signal-to-noise (SNR) than SBS microscopy and continuous-wave Brillouin microscopy, even with reduced average power exposure. Experimental results demonstrate a shot noise-limited SNR exceeding 1000 with precise mapping of cellular features. The interlaced boxcar method effectively retrieves the SBS signal, while optimizing pulse width and peak power further enhances optical power efficiency. This pulse scheme offers improved precision and reduced laser power exposure compared to spontaneous Brillouin and continuous wave (cw) SBS approaches.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Desmond M. Chow and Seok-Hyun Yun "Enhancing signal-to-noise ratio in stimulated Brillouin microscopy with low duty cycle nanosecond pulses", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540D (12 March 2024); https://doi.org/10.1117/12.2692239

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