All-optical observation and control of communication between neuronal cell-types during behavior via structure-guided design of channelrhodopsins

Masatoshi Inoue; Karl Deisseroth

doi:10.1117/12.3002841

13 March 2024 All-optical observation and control of communication between neuronal cell-types during behavior via structure-guided design of channelrhodopsins

Masatoshi Inoue, Karl Deisseroth

Author Affiliations +

Proceedings Volume 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; 1286203 (2024) https://doi.org/10.1117/12.3002841
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Understanding the nature and role of communication between distinct cellular populations in the brain will require simultaneous measurement and control of activity within those populations during behavior. Current optogenetic tools, present limitations such as spectral incompatibility with sensors and modest efficacy. To address these issues, we engineered novel red-shifted high potent cation-selective channelrhodopsin, rsChRmine, and K⁺-selective channelrhodopsins (KCRs), KALIs, with enhanced K⁺ selectivity based on our cryo-EM structures. By integrating these new opsins with multiple genetically-encoded Ca²⁺ indicators, we can selectively control specific neural circuits while simultaneously observing the responses of other elements within the same network. We applied this method to the mPFC of freely-behaving mice, quantifying dynamic information transmission between excitatory and inhibitory populations. Together, this work lays the foundation for new kinds of investigation into brain function and dysfunction.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Masatoshi Inoue and Karl Deisseroth "All-optical observation and control of communication between neuronal cell-types during behavior via structure-guided design of channelrhodopsins", Proc. SPIE 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, 1286203 (13 March 2024); https://doi.org/10.1117/12.3002841

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