Modern age of pathological sciences: fluorescence microscopy for proficient treatment

Azhar Javed Satti; Isma Javed; Muhammad Zubair; Muhammad Qasim Mehmood

doi:10.1117/12.3022061

18 June 2024 Modern age of pathological sciences: fluorescence microscopy for proficient treatment

Azhar Javed Satti, Isma Javed, Muhammad Zubair, Muhammad Qasim Mehmood

Proceedings Volume 13009, Clinical Biophotonics III; 130090J (2024) https://doi.org/10.1117/12.3022061
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

In light microscopy, achieving uniform lighting throughout the field of view is essential to obtain clear images. However, thicker samples commonly suffer from image blur due to out-of-focus illumination, notably raised in fluorescence microscopy because dye molecules are activated across all planes, including those out of focus. Confocal-type fluorescence microscopy efficiently addresses this obstacle by reducing out-of-focus light, thereby permitting high-quality imaging in thick tissues. By focusing the light and detector on a diffraction-limited point and monitoring the material point by point, confocal microscopes may obtain full pictures with optical sectioning abilities for 3D reconstruction. With numerous varieties offering distinct advantages and limits, confocal microscopes serve as important tools for accurate imaging and analysis across different sectors. This article gives a concise review of confocal fluorescence microscopy.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Azhar Javed Satti, Isma Javed, Muhammad Zubair, and Muhammad Qasim Mehmood "Modern age of pathological sciences: fluorescence microscopy for proficient treatment", Proc. SPIE 13009, Clinical Biophotonics III, 130090J (18 June 2024); https://doi.org/10.1117/12.3022061

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