A phase contrast cell microscopic image enhancement algorithm based on sliding window and multi-scale Gaussian filtering

Bo Liu; Yongzhao Du; Tong Yang; Haixin Chen; Xiaolin Wu; Xin Chen

doi:10.1117/12.3026379

8 April 2024 A phase contrast cell microscopic image enhancement algorithm based on sliding window and multi-scale Gaussian filtering

Bo Liu, Yongzhao Du, Tong Yang, Haixin Chen, Xiaolin Wu, Xin Chen

Author Affiliations +

Proceedings Volume 13090, International Conference on Computer Application and Information Security (ICCAIS 2023); 130903D (2024) https://doi.org/10.1117/12.3026379
Event: International Conference on Computer Application and Information Security (ICCAIS 2023), 2023, Wuhan, China

Abstract

Aiming at the issues of low contrast, weak edges, and poor clarity of phase contrast cell microscopic images, which lead to the inability to observe cell features clearly, a phase contrast cell microscopic image enhancement algorithm based on sliding window and multi-scale Gaussian filtering is proposed. Firstly, an adaptive sliding window grayscale enhancement method is proposed to enhance the contrast and edge features of phase contrast cell microscopic images. Secondly, in the multi-scale feature fusion stage, the enhanced phase contrast cell microscopic images are filtered with a guided filter to suppress some noise and impurities. And the multi-scale Gaussian filtering weighted fused method is proposed to extract the structure image that contains edge and detail features. Finally, in the structure features enhancement stage, the guided filtered phase contrast cell microscopic images are differential of the structure features constrained by the adaptive enhancement factor to obtain the enhanced phase contrast cell microscopic images. The experimental results show that the proposed phase contrast cell microscopic image enhancement algorithm based on sliding window and multi-scale Gaussian filtering is superior to the existing traditional methods in subjective and objective image quality evaluation, and effectively improves the contrast and enhances the detailed features and edges of the cells in the phase contrast cell microscopic images.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Bo Liu, Yongzhao Du, Tong Yang, Haixin Chen, Xiaolin Wu, and Xin Chen "A phase contrast cell microscopic image enhancement algorithm based on sliding window and multi-scale Gaussian filtering", Proc. SPIE 13090, International Conference on Computer Application and Information Security (ICCAIS 2023), 130903D (8 April 2024); https://doi.org/10.1117/12.3026379

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KEYWORDS

Image enhancement

Phase contrast

Windows

Image filtering

Tunable filters

Gaussian filters

Feature extraction

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