Low-cost Fourier ptychographic microscopy for neuroimaging applications

Stephan Bannikov; Paul Gaston; Daryl Preece

doi:10.1117/12.3028924

2 October 2024 Low-cost Fourier ptychographic microscopy for neuroimaging applications

Stephan Bannikov, Paul Gaston, Daryl Preece

Proceedings Volume 13112, Optical Trapping and Optical Micromanipulation XXI; 131120C (2024) https://doi.org/10.1117/12.3028924
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Conference Poster

Abstract

For a fluorescence microscopy system, a set-up often costs upwards of one hundred thousand dollars, if not multiple hundreds. This makes research inaccessible to any institutions except those with the highest level of funding. Our research addresses the challenges of high-magnification fluorescence microscopy by using low-cost off-the-shelf components to create a fluorescence imaging system. We use a cost-effective widefield system for neuroimaging using an OLED array via an Arduino microcontroller and a custom-made image processing algorithm. The OLEDs enable fluorescence microscopy with their green and blue emissions having significant overlap with the absorption spectrums for the Alexa Fluor 532 and FITC fluorescent probes. We use this system for Fourier ptychography and leverage image processing algorithms to achieve high-resolution images by combining low-resolution images within the Fourier plane. A phase retrieval algorithm and a regression model are utilized to find optimal coefficients for our transform matrices. This project is promising to democratize neuroimaging, especially in resource-constrained settings with affordable costs and portability. It is small-scale, 6’x8’x12’, and compatible with any MATLAB system, thereby allowing even further reach into under-served areas.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Stephan Bannikov, Paul Gaston, and Daryl Preece "Low-cost Fourier ptychographic microscopy for neuroimaging applications", Proc. SPIE 13112, Optical Trapping and Optical Micromanipulation XXI, 131120C (2 October 2024); https://doi.org/10.1117/12.3028924

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available