Bio-Raman spectral analysis of living cells

Shin-ichi Morita; Mohamed M. Abdel-Galeil; Jianhai He; Kazumasa Ohashi; Yuki Umeda; Satoshi Yamaguchi

doi:10.1117/12.3033232

31 December 2024 Bio-Raman spectral analysis of living cells

Shin-ichi Morita, Mohamed M. Abdel-Galeil, Jianhai He, Kazumasa Ohashi, Yuki Umeda, Satoshi Yamaguchi

Author Affiliations +

Proceedings Volume 13487, Optics and Photonics International Congress 2024; 1348706 (2024) https://doi.org/10.1117/12.3033232
Event: Optics and Photonics International Congress, 2024, Yokohama, Japan

Abstract

We sought effective height positions at the center part of the nucleus of a living HeLa cell using a bio-Raman microscope, expecting to define the internal state of the living cell. Bio-Raman Non-negative matrix factorization (bio-Raman NMF) was used to provide meaningfully decomposed spectra from complicated Raman spectra of living cells. The heightdependent Raman spectra were successfully and meaningfully factorized into 3 Raman spectral components. All the 3 components seemed effective to define the internal state of a cell.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Shin-ichi Morita, Mohamed M. Abdel-Galeil, Jianhai He, Kazumasa Ohashi, Yuki Umeda, and Satoshi Yamaguchi "Bio-Raman spectral analysis of living cells", Proc. SPIE 13487, Optics and Photonics International Congress 2024, 1348706 (31 December 2024); https://doi.org/10.1117/12.3033232

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