Dynamic light-scattering study of monodisperse collagen in the less-dilute regime

Tadakazu Maeda; Michiho Takasaki-Ohsita; Keiko Satoh; Mayumi Kimura

doi:10.1117/12.148372

21 July 1993 Dynamic light-scattering study of monodisperse collagen in the less-dilute regime

Tadakazu Maeda, Michiho Takasaki-Ohsita, Keiko Satoh, Mayumi Kimura

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148372
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Physicochemical properties of monodisperse type I collagen were studied using the dynamic light scattering and analytical ultra-centrifugation techniques. The measured osmotic second virial coefficient A₂ is much smaller than the excluded volume limit calculated from the length L, diameter b and molecular weight M of collagen. The strength k_D of the concentration dependence of the mutual diffusivity ^*D_G of collagen is negative in the sign and large in the magnitude. The relationship k_D equals 2A₂M - v - k_(zetz ) does not hold for collagen, while it holds for fd virus and poly((gamma) - benzyl L-glutamate), where v is the partial specific volume, and k_(zetz ) the strength of the concentration dependence of the friction coefficient (zetz) . We conclude from these three lines of evidence that water is a poor solvent of collagen and that there must be very weak attractive interactions among collagen filaments which can easily be destroyed by external perturbations.

Citation Download Citation

Tadakazu Maeda, Michiho Takasaki-Ohsita, Keiko Satoh, and Mayumi Kimura "Dynamic light-scattering study of monodisperse collagen in the less-dilute regime", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148372

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