Monte Carlo modelization of laser-induced fluorescence signals in turbid media: determination of original fluorescence spectra

Eric Tinet; Sigrid Avrillier

doi:10.1117/12.151199

27 August 1993 Monte Carlo modelization of laser-induced fluorescence signals in turbid media: determination of original fluorescence spectra

Eric Tinet, Sigrid Avrillier

Author Affiliations +

Proceedings Volume 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods; (1993) https://doi.org/10.1117/12.151199
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In this theoretical paper we study the alterations of the laser induced fluorescence spectra as a function of the spectral variations of the absorption factor μ_a(λ), the scattering factor μ_s(λ) and of the mean cosine of the scattering angle g(λ) of the medium. The calculations of the signal received by the detector are based on a Monte Carlo simulation. All media are homogeneous, with infinite length and width, depth is assumed to be infinite. The total detected fluorescence signal S(λ) may be described by: S(λ)= F(λ_e,λ) . M(μ_a(λ_e),μ_s(λ_e),g(λ_e),μ_a(λ),μ_s(λ),g(λ)) where F is proportional to the original fluorescence spectrum and M a function of the optical and geometrical properties of the medium. In order to show the importance of the scattering, absorption and anisotropy spectral dependence, we have computed M for ideal experiments. Each coefficient μ_a, μ_s and g is alternately considered to be wavelength dependent while the others are considered to be constant. Consequently, each calculation provides a different distorted transfer function M(λ_e,λ) showing the influence of the spectral dependence of each coefficient. This signal is also different if the wavelength of the excitation light is changed.

Citation Download Citation

Eric Tinet and Sigrid Avrillier "Monte Carlo modelization of laser-induced fluorescence signals in turbid media: determination of original fluorescence spectra", Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); https://doi.org/10.1117/12.151199

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