Scanning tunneling microscopy, atomic force microscopy, and surface analysis methods for the investigation of biomolecule structure at a solid surface

Carol E. Rabke-Clemmer; Lisa A. Wenzler; Thomas P. Beebe Jr.

doi:10.1117/12.146717

24 June 1993 Scanning tunneling microscopy, atomic force microscopy, and surface analysis methods for the investigation of biomolecule structure at a solid surface

Carol E. Rabke-Clemmer, Lisa A. Wenzler, Thomas P. Beebe Jr.

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146717
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We present results of STM and AFM investigations of coated and uncoated samples containing plasmid DNA, chemically modified DNA and tobacco mosaic virus. These specimens were adsorbed by a variety of methods onto low Miller index gold single crystals, co-evaporated films, and mica substrates. Some of the samples were prepared and transferred into an ultrahigh vacuum chamber for further treatment and analysis by Auger electron spectroscopy (AES) and electron spectroscopy for chemical analysis (ESCA or XPS) in an effort to investigate various methods for depositing chemically modified DNA onto gold and mica substrates. These results will be discussed in the context of corroborating STM and AFM image results with the established techniques of AES and ESCA. It is potentially beneficial to make certain chemical modifications to the surfaces and the DNA for two purposes: to aid in adsorption of the molecule to the substrate and to provide a label for the electron spectroscopy verification studies. We hope to enhance the molecule-substrate interactions in such a way as to make the imaging of the biomolecules such as DNA more reproducible and less prone to artifacts.

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Carol E. Rabke-Clemmer, Lisa A. Wenzler, and Thomas P. Beebe Jr. "Scanning tunneling microscopy, atomic force microscopy, and surface analysis methods for the investigation of biomolecule structure at a solid surface", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146717

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KEYWORDS

Mica

Scanning tunneling microscopy

Molecules

Magnesium

Atomic force microscopy

Spectroscopy

Chemical analysis

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