Fluorescence resonance energy transfer measurements as a tool to detect fusion and drug exchange in liposomal suspensions

Rolf Hilfinker; Annette Willi; Ute Isele; Peter van Hoogevest

doi:10.1117/12.168689

1 March 1994 Fluorescence resonance energy transfer measurements as a tool to detect fusion and drug exchange in liposomal suspensions

Rolf Hilfinker, Annette Willi, Ute Isele, Peter van Hoogevest

Author Affiliations +

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168689
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Zinc-(II)-phthalocyanine (ZnPc) is a drug that can potentially be used in photodynamic therapy. A promising formulation approach for the strongly hydrophobic ZnPc is to embed the molecule in liposomes. CGP 55847 is such a liposomal ZnPc formulation. From our data, we could conclude that approximately one quarter of the drug molecules were only loosely bound to the liposomes and exchanged between them with a relaxation time of about 15 min. Energy transfer measurements before and after the lyophilization process revealed that the liposomes were stable when suspended in a 10% lactose solution, i.e. no fusion of liposomes took place. In aqueous solution, however, lyophilization induced fusion of liposomes to a small extent with a concomitant increase in size.

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Rolf Hilfinker, Annette Willi, Ute Isele, and Peter van Hoogevest "Fluorescence resonance energy transfer measurements as a tool to detect fusion and drug exchange in liposomal suspensions", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168689

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