Optical methods of investigation of the protein Langmuir: Blodgett films

Oleg I. Lobanov; T. B. Dubrovsky; Alexander P. Savitsky; D. E. Akindinov; Ilya V. Alexeev; V. V. Savransky

doi:10.1117/12.167471

1 February 1994 Optical methods of investigation of the protein Langmuir: Blodgett films

Oleg I. Lobanov, T. B. Dubrovsky, Alexander P. Savitsky, D. E. Akindinov, Ilya V. Alexeev, V. V. Savransky

Proceedings Volume 2082, Quantification and Localization Using Diffuse Photons in a Highly Scattering Medium; (1994) https://doi.org/10.1117/12.167471
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The structure of multilayer Langmuir-Blodgett (LB) films made of protein (Bovine serum albumine)-co-proporphyrin(III) conjugate was investigated by means of fluorescence microscope coupled with the time-correlated single photon counting registration system. The map of coproporphyrin fluorescence from the surface of protein LB-films was measured with picosecond time resolution and spatial resolution 15 micrometers . The sample area 240 X 240 micrometers was irradiated step by step by a focused beam of Nd:YAG laser with an accumulation time of 3 sec per point. There were two different kinds of samples: those with regular and those with irregular distribution of fluorescent labels over the sample.

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Oleg I. Lobanov, T. B. Dubrovsky, Alexander P. Savitsky, D. E. Akindinov, Ilya V. Alexeev, and V. V. Savransky "Optical methods of investigation of the protein Langmuir: Blodgett films", Proc. SPIE 2082, Quantification and Localization Using Diffuse Photons in a Highly Scattering Medium, (1 February 1994); https://doi.org/10.1117/12.167471

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