Tissue diagnosis by means of endogenous fluorophores

Wolfgang Lohmann; Thomas Dreyer; M. Nilles; Rainer M. Bohle; Wolf-Bernhard Schill; Hiltrud Glanz; Andreas Fryen; Gisela Horn; Beate Pollich

doi:10.1117/12.198703

4 January 1995 Tissue diagnosis by means of endogenous fluorophores

Wolfgang Lohmann, Thomas Dreyer, M. Nilles, Rainer M. Bohle, Wolf-Bernhard Schill, Hiltrud Glanz, Andreas Fryen, Gisela Horn, Beate Pollich

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198703
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

In vitro or in situ single spot or area excitation of human tissue with either 337 nm or 365 nm results in a fluorescence spectrum at about 470 nm. Since in most tissue samples the spectra obtained look alike and differ in intensity only, the 2D tomographical distribution of the fluorescence intensity was also investigated. It could be shown that the fluorescence images of unfixed and unstained cryosections match the histological images (HE stained cryosections). They exhibit a fluorescence pattern unique for the different types of diseases investigated (basalioma, melanoma, nevi, psoriasis, seborrheic keratosis). Additional information can be obtained by elastica van Gieson stained images and by illuminating the HE stained cryosections with 365 nm. Since the cryosections can be prepared and evaluated in less than 5 minutes, this technique might be used as a fast cut technique for determining e.g. the diagnosis of a biopsy sample, also during surgery.

Citation Download Citation

Wolfgang Lohmann, Thomas Dreyer, M. Nilles, Rainer M. Bohle, Wolf-Bernhard Schill, Hiltrud Glanz, Andreas Fryen, Gisela Horn, and Beate Pollich "Tissue diagnosis by means of endogenous fluorophores", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198703

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